Lab Manager - Cancer Center - 141525

The Moores Cancer Center (MCC) is one of just 57 NCI-designated Comprehensive Cancer Centers in the United States and the only one in San Diego County. As a consortium cancer center, it is a collaborative partnership between the UCSD: encompassing 28 departments, 6 schools School of Medicine, Skaggs School of Pharmacy and Pharmaceutical Sciences, School of Public Health, Jacobs School of Engineering, School of Biological Sciences & the School of Physical Sciences), UCSD Health oncology hospitals and clinics; the basic and public health research and outreach of San Diego State University (SDSU), and the basic and translational research of the La Jolla Institute of Immunology (LJI). These various programs and units are all dedicated to fulfilling the Moores Cancer Center's mission of reducing cancer's burden. As such, it ranks among the top centers in the nation conducting the continuum of cancer research, providing advanced patient care, and serving the community through outreach and education programs. As a top-ranking, future-oriented organization, we offer challenging career opportunities in a fast-paced and innovative environment. Moores Cancer Center follows a progressive philosophy of career-path development for its employees including opportunities for cross-training, professional development, and progressive responsibility.

MCC's mission is to transform cancer care in our catchment area and beyond by driving exceptional scientific discoveries and innovations in prevention, detection, care, and training. MCC will make a global impact on improving health by reducing cancer burden through accelerateddiscovery and translation, compassionate and interdisciplinary care, education and community engagement, with the foundation of our core values - Excellence, Innovation and Risk-Taking, Collaboration, Diversity and Service.

Independently design, perform and analyze experiments directed at developing novel protein-based cancer chemotherapeutic agents for the treatment of cancers. This includes: a) design and construction of novel types of vectors for cellular engineering; b) development of novel techniques for the production of proteins in human cell systems, and selection of stable clones; c) creation of complex chromatographic systems to develop better ways to purify proteins from cell supernates or lysates; d) interpretation of analytic tools for the characterization of novel proteins including HPLC, FPLC, FACS, IHC and ICC and interpretation of results; e) development of cellular and molecular biology techniques to characterize the pharmacodynamics of drugs such as in vitro cytotoxicity assays; f) independently design and manage execution of efficacy, pharmacokinetic and pharmacodynamic studies in mice and rats; g) make innovative contributions to experimental design and methodology by reviewing published literature and online resources; h) provide training and assistance to staff scientists, post-doctoral fellows and student on cutting edge techniques in the field of experimental therapeutics.

• Advanced theoretical knowledge and/or Bachelor's Degree in virology, biochemistry, cellular and molecular biology. Experience with viral vector construction, purification, and infection and transfections with various DNA plasmids. Knowledge and experience with DNA and RNA purification.

• Experience with the design of vectors for the production of recombinant protein and the molecular biology techniques for their construction and functional assessment.

• Experience with biochemical techniques including gel electrophoresis, measurement of protein concentrations, preparation of buffers, operation of spectrophotometers and other routine laboratory equipment.

• Proven ability to contribute to original project development ideas, experimental design and strategies, as well as their implementation into a variety of research projects.

• Knowledge of techniques for large-scale highly reproducible production of recombinant proteins in bacteria and mammalian cell systems.

• Experience with modern protein purification techniques including HPLC, FLPC, affinity resins and antibody-based purification systems.

• Knowledge of, and experience with physical biologic techniques for the characterization of recombinant proteins including binding affinity.

• Understanding of principles that guide development of protein therapeutics such as antibody-drug conjugates.

• Experience with the physical and functional characterization of recombinant proteins.

• Experience with culturing of bacterial and mammalian cells and their preservation.

• Basic understanding of the principles of pharmacokinetics.

• Strong communication skills (both verbal and written) needed to interact professionally and effectively in the work environment. Effectively and accurately document research procedures.

• Knowledge and skill to conduct library research. Ability to read, comprehend and discuss research materials. Skill to critically analyze the information, read protocols and be able to design and apply new techniques independently.

• Experience using computer software including Microsoft Word and Excel.

• Experience analyzing and interpreting data. Experience designing new experiments as new techniques are established. Knowledge of and experience in testing research hypotheses.

• Demonstrated experience with cell and molecular biology techniques including tissue culture, DNA and RNA isolation and quantitation, PCR, qRT-PCR, construction of vectors, western blots, transfection/infection, flow cytometry, microscopy, in vitro cell survival assays, bacterial transformation, plasmid preparation, protein isolation and purification.

• Demonstrated experience with enzyme assays, drug sensitivity assays in tissue culture, pharmacokinetics, production of recombinant proteins in bacteria, production of recombinant protein in CHO cells, and/or proteomic analysis.

• Employment is subject to a criminal background check.

• Must be able to work various hours and locations based on business needs. Must be willing to occasionally work late and/or evenings or weekends as needed.

• Must be willing and able to work with biohazardous materials at BL3 level.